Info-enriched characterization of cell populations. Morphological facts from photos can increase to your richness of flow cytometry details. Such as, the determine displays an usually conventional apoptosis assay employing Annexin V and PI, including cell imaging to characterize cells in Each individual populace to expose morphologically distinctive capabilities. These insights could not are actually attained from multiplex staining by itself.
As samples are acquired on the Attune CytPix Flow Cytometer, the high-pace brightfield digital camera captures and retailers visuals of detected events in a fee around 6,000 photos for every second, according to the flow rate and picture size. For better versatility, Attune Cytometric Software program permits you to adjust the impression capture frequency as necessary and choose a certain gate for picture seize, if essential.
Combine acoustic concentrating technology with superior-parameter Assessment for flexible spectral unmixing and standard workflows.
Find new insights into your experimental knowledge by visualizing populations using combined fluorescence and morphology info. Our computer software interface wizards allow downsampling, concatenation (merged information) and dimensionality equipment which might be created in to the program for an intuitive, streamlined information analysis encounter.
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Here, an experienced user has gated singlets confidently. Following analyzing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate is made up of a lot more than four% aggregates.
Pair your Attune flow cytometer having an autosampler for increased workflow performance, and increase robotic integration for maximum throughput.
Regular image high-quality even at large throughput Acoustic focusing plus a higher-pace camera Merge to graphic these T cells persistently at low or significant flow rates. Conveniently modify emphasis and camera options to meet experimental specifications.
The Attune NxT cytometer would be the responsible workhorse instrument your lab can rely on for consistent and trusted effectiveness. The Attune CytPix model contains a large-velocity brightfield digital camera and AI-pushed picture Investigation software package, with latest updates which include equipment Discovering equipment and an intuitive user interface, enabling Improved knowledge precision, precision, and faster insights into sample biology.
Maybe most significantly, these gatherings consist of cells of Plainly different phenotypes which could lead on to incorrect conclusions relating to double beneficial gatherings (specifically in rare populations).
Seem waves allow specific mobile alignment, leading to Excellent information quality and ~10X speedier sample flow prices.
We Earlier shown the power of imaging CAR-T/Ramos cell interactions. Permit’s look at just the inhabitants of best desire, the double constructive gatherings, To find out more. We can easily now use extended morphological parameters (in this article, circularity and skewness of intensity) to more look at the attributes of those populations and refine gating on these functions, increasing data robustness.
Developed-in dimensionality reduction applications improved resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm anticipated inhabitants resolution in lysed complete human blood. Consumer can view situations from Each individual cluster for Visible affirmation of mobile phenotypes.
No wash/no lyse leukocyte analysis with imaging. Cells ended up obtained from 24-hour-outdated blood by dilution in one mM EDTA ( Specialist UX/UI allows customers to train AI products for expanded purposes. (A) The “Teach Model” feature presents annotation applications and actual-time design coaching precision.
Gating on these extended parameters means that you can promptly and precisely establish populations of curiosity and confirm gating tactic with little or no handbook evaluation. The Attune software program presents entry to state-of-the-art device Mastering Examination applications in an expertly intended consumer interface, making it extremely simple to use.
The Attune Xenith cytometer is often a large-parameter spectral analyzer, delivering both of those spectral unmixing and conventional flow abilities for complete and versatile cell analysis.
Agilent Benefit Assure ensures you a minimum of 10 years of instrument use within the day you purchase. Usually, we will credit history you While using the residual value of the technique toward an upgraded design.
What seemed to be a homogeneous population is actually extra diverse—an insight that needs more info to be deemed when interpreting outcomes.